Spatial Light Modulation as a Versatile Tool for Sensorless Aberration Correction in Fluorescence Microscopy and Nanoscopy

نویسندگان

  • André Klauss
  • Florian Conrad
  • Carsten Hille
چکیده

Spatial (phase-only) light modulators (SLMs) have successfully been used for different tasks in microscopy, one prominent example being the doughnut beam shaping in STED nanoscopy [1]. Once implemented into the laser beam path of a microscopic imaging system, its free programmability makes the SLM a versatile instrument for multiple uses. Aberration correction in such a retrofitted system, for example, is readily realized by introducing compensating phase functions into the pupil [2,3].

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Semidefinite programming for model-based sensorless adaptive optics.

Wavefront sensorless adaptive optics methodologies are widely considered in scanning fluorescence microscopy where direct wavefront sensing is challenging. In these methodologies, aberration correction is performed by sequentially changing the settings of the adaptive element until a predetermined image quality metric is optimized. An efficient aberration correction can be achieved by modeling ...

متن کامل

gSTED Microscopy with an OPSL: Cutting Edge Super-Resolution Breaking the diffraction limit with a CW OPSL

In order to probe cell biology at the molecular level and correlate single molecule activity with macroscopic cellular behavior in real-time, biologists need farfield microscopy techniques with the highest possible spatial as well as temporal resolution. Driven by this need, the use of lasers in confocal microscopy has taken fluorescence microscopy to the diffraction limit, which was long consi...

متن کامل

Lens-based fluorescence nanoscopy.

The majority of studies of the living cell rely on capturing images using fluorescence microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution in the optical microscope: structural and molecular details much finer than about half the wavelength of visible light (~200 nm) could not be visualized, imposing significant limitations on this otherwise so pro...

متن کامل

Coherence-Gated Sensorless Adaptive Optics Multiphoton Retinal Imaging

Multiphoton microscopy enables imaging deep into scattering tissues. The efficient generation of non-linear optical effects is related to both the pulse duration (typically on the order of femtoseconds) and the size of the focused spot. Aberrations introduced by refractive index inhomogeneity in the sample distort the wavefront and enlarge the focal spot, which reduces the multiphoton signal. T...

متن کامل

SLM-based Microscopy

We present a microscope that has the capability of adapting to various object characteristics by applying different filters in the Fourier plane of the imaging path. To this end we use a spatial light modulator and display different computer generated holograms. This allows us to realize several phase contrast techniques, dynamic aberration correction, pseudostereoscopic microscopy and confocal...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2017